Gonadotropin-Inhibitory Hormone Promoter-Driven Enhanced Green Fluorescent Protein Expression Decreases During Aging in Female Rats
Tomoko Soga, Takashi Kitahashi, Iain J Clarke, Ishwar S Parhar
ENDOCRINOLOGY | OXFORD UNIV PRESS INC | Published : 2014
Gonadotropin-inhibitory hormone (GnIH) neurons project to GnRH neurons to negatively regulate reproductive function. To fully explore the projections of the GnIH neurons, we created transgenic rats carrying an enhanced green fluorescent protein (EGFP) tagged to the GnIH promoter. With these animals, we show that EGFP-GnIH neurons are localized mainly in the dorsomedial hypothalamic nucleus (DMN) and project to the hypothalamus, telencephalon, and diencephalic thalamus, which parallels and confirms immunocytochemical and gene expression studies. We observed an age-related reduction in c-Fos-positive GnIH cell numbers in female rats. Furthermore, GnIH fiber appositions to GnRH neurons in the p..View full abstract
Awarded by Monash University Sunway Campus
Awarded by Ministry of Higher Education, Malaysia
This work was supported by research grants from Monash University Sunway Campus (TS01-10 to T.S. and I.S.P.) and Fundamental Research Grant Scheme 2011 from the Ministry of Higher Education, Malaysia (FRGS/1/2011/ST/MUSM/02/1 to T.S. and I.S.P.).