Journal article

Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function

Amna R Tariq, Muhammad Shahab, Iain J Clarke, Alda Pereira, Jeremy T Smith, Saeed-ul-Hasan Khan, Javeria Sultan, Sehrish Javed, Tayyaba Anwar

CENTRAL EUROPEAN JOURNAL OF BIOLOGY | DE GRUYTER POLAND SP Z O O | Published : 2013

Abstract

Background: Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures: Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 μ thick sections. Using gene specif..

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Funding Acknowledgements

This piece of research work was funded by the Higher Education Commission of Pakistan (HEC) and Amna Rashid Tariq was an HEC fellowship holder. There was no conflict of interest among authors that could be perceived as prejudicing the impartiality of the research work.