Journal article

Seasonal and Cyclical Change in the Luteinizing Hormone Response to Kisspeptin in the Ewe

Jeremy T Smith, Sofie NH Saleh, Iain J Clarke



BACKGROUND: Kisspeptins are recognized as important stimulators of gonadotropin-releasing hormone (GnRH) and gonadotropin secretion. In sheep, the nonbreeding (anestrous) period is characterized by reduced Kiss1 mRNA expression in the hypothalamus. The aim of the present study was to determine whether the response to kisspeptin treatment varies between the breeding and nonbreeding seasons. METHODS: Corriedale ewes were treated intravenously with murine kisspeptin-10 (submaximal dose: 20 microg or maximal dose: 250 microg) or GnRH (250 ng) during either the anestrous period or during the breeding season at the luteal, mid-follicular, or late-follicular phase of the estrous cycle. RESULTS: Kis..

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University of Melbourne Researchers


Awarded by National Health and Medical Research Council of Australia

Awarded by Peter Doherty Fellowship

Funding Acknowledgements

We thank Mr. B. Doughton, Ms. L. Morrish, Ms. E. Leeson, and Ms. J. Thomas for technical assistance. Kisspeptin [murine C-terminal Kiss1 decapeptide (110-119)-NH2] was kindly supplied by Professor Alain Caraty (Universite de Tours, Nouzilly, France). This work was supported by the National Health and Medical Research Council of Australia (384124). Jeremy T. Smith is supported by a Peter Doherty Fellowship (384362) and a recipient of an Endocrine Society of Australia Postdoctoral Award.