Journal article

Seasonal changes in the inputs to gonadotropin-releasing hormone neurones in the ewe brain: An assessment by conventional fluorescence and confocal microscopy

S Pompolo, A Pereira, T Kaneko, IJ Clarke

JOURNAL OF NEUROENDOCRINOLOGY | WILEY | Published : 2003

Abstract

The seasonal pattern of breeding in sheep offers an opportunity to examine plasticity of neuronal inputs to gonadotropin-releasing hormone (GnRH) neurones. We used conventional fluorescence microscopy and confocal microscopy to compare the extent of input to GnRH neurones from various neuropeptide/neurotransmitter systems in ewes during the breeding and anestrous seasons. Using double-labelling immunohistochemistry, we counted close appositions between GnRH cells and varicosities that were immunoreactive for either glutamic acid decarboxylase (GAD; for gamma-amino butyric acid-GABA-neurones), dopamine beta hydroxylase (DBH; for noradrenergic neurones), vesicular glutamate transporter-1 (VGlu..

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University of Melbourne Researchers