Journal article

CIRCULATING HALF-LIVES OF FOLLICLE-STIMULATING-HORMONE AND LUTEINIZING-HORMONE IN PITUITARY EXTRACTS AND ISOFORM FRACTIONS OF OVARIECTOMIZED AND INTACT EWES

DM ROBERTSON, LM FOULDS, RC FRY, JT CUMMINS, I CLARKE

ENDOCRINOLOGY | ENDOCRINE SOC | Published : 1991

Abstract

Previous studies have shown that the circulating half-life (t 1/2) of serum FSH in ewes after hypophysectomy (HPX) increased 10-fold after ovariectomy (OVEX). The basis for this difference was examined in this study by determining the circulating half-life of serum FSH and LH in HPX ewes after administration of pituitary extracts and gonadotropin isoform fractions. High-speed supernatants of pituitaries from gonadal-intact and OVEX ewes were fractionated by electrofocusing in sucrose gradients and based on the pI distribution of FSH and LH divided into four pools, pH 4.3-4.8, 4.8-5.55, 5.8-6.7, and 6.7-10. These extracts were administered by iv bolus injection to HPX gonadal-intact ewes and ..

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