Journal article

MEASUREMENT OF GROWTH HORMONE-RELEASING HORMONE AND SOMATOSTATIN IN HYPOTHALAMIC-PORTAL PLASMA OF UNANESTHETIZED SHEEP - SPONTANEOUS SECRETION AND RESPONSE TO INSULIN-INDUCED HYPOGLYCEMIA

LA FROHMAN, TR DOWNS, IJ CLARKE, GB THOMAS

JOURNAL OF CLINICAL INVESTIGATION | ROCKEFELLER UNIV PRESS | Published : 1990

Abstract

To elucidate the role of growth hormone (GH)-releasing hormone (GRH) and somatostatin (SRIH) in the regulation of the growth hormone (GH) secretory pattern, we collected portal blood from five unanesthetized ovariectomized ewes for repeated measurements of GRH and SRIH simultaneous with those of peripheral GH. Hormones were measured at 10-min intervals for 5.5 h and their interrelationships analyzed. Mean portal GRH was 20.4 +/- 6.7 (SD) pg/ml and the estimated overall secretion rate was 13 pg/min. GRH secretion was pulsatile with peaks of 25-40 pg/ml and a mean pulse interval of 71 min. Mean portal SRIH was 72 +/- 33 pg/ml and the estimated overall secretion rate was 32 pg/min. SRIH secreti..

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