Journal article

The distribution of cells containing estrogen receptor-alpha (ER alpha) and ER beta messenger ribonucleic acid in the preoptic area and hypothalamus of the sheep: Comparison of males and females

CJ Scott, AJ Tilbrook, DM Simmons, JA Rawson, S Chu, PJ Fuller, NH Ing, IJ Clarke

ENDOCRINOLOGY | ENDOCRINE SOC | Published : 2000

Abstract

We have used in situ hybridization to compare the distributions of estrogen receptor alpha (ERalpha) and ERbeta messenger RNA (mRNA)-containing cells in the preoptic area and hypothalamus of ewes and rams. Perfusion-fixed brain tissue was collected from luteal phase ewes and intact rams (n = 4) during the breeding season. Matched pairs of sections were hybridized with sheep-specific, 35S-labeled riboprobes, and semiquantitative image analysis was performed on emulsion-dipped slides. A number of sex differences were observed, with females having a greater density of labeled cells than males (P < 0.001) and a greater number of silver grains per cell (P < 0.01) in the ventromedial nucleus for b..

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