Journal article

USE OF AN ENZYME-LINKED IMMUNOSORBENT-ASSAY IN A BOVINE BRUCELLOSIS ERADICATION PROGRAM

C CARGILL, K LEE, I CLARKE

AUSTRALIAN VETERINARY JOURNAL | AUSTRALIAN VETERINARY ASSN | Published : 1985

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed and was compared with the complement fixation test (CFT) in a bovine brucellosis eradication program. The ELISA detected significantly more reactors than the CFT in both strain 19 vaccinated infected herds (1.79% versus 1.14%) and non-vaccinated infected herds (4.2% versus 3.59%) but not in either vaccinated or non-vaccinated brucella-free herds. The specificity for both tests in brucella-free herds was greater than 0.998. The specificity and sensitivity of the ELISA were compared with those of 3 other tests (the Rose Bengal test; the indirect haemolysis test [IHLT] and the CFT) on serum from 151 animals cultured at slaughter. The ca..

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University of Melbourne Researchers