Journal article

Expression of Genes for Appetite-Regulating Peptides in the Hypothalamus of Genetically Selected Lean and Fat Sheep

Chantacha Anukulkitch, Alexandra Rao, Alda Pereira, John McEwan, Iain J Clarke



BACKGROUND/AIMS: The aim was to determine whether genetic selection of sheep for body composition could be accounted for by changes in the level of expression of genes for appetite-regulating peptides in the hypothalamus. We examined gene expression in the hypothalamus of genetically lean, normal and fat ewes (n = 5/group). METHODS: Plasma growth hormone (GH) and metabolic indicators were measured and gene expression in brains was quantified by in situ hybridization. RESULTS: Body weight and voluntary food intake (VFI) were similar between groups, but lean and fat animals respectively had low and high indices of adiposity. GH levels were higher in lean and fat animals than in controls. In th..

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University of Melbourne Researchers


Funding Acknowledgements

We thank Margaret Blackberry and Dominique Blache, University of Western Australia for leptin assays and Mr. Gordon Greer for animal husbandry and assistance during animal experimentation. We are grateful to Daqing Qu, Harvard Medical School, for the MCH probe and Steven Sabol, NHBLI, Bethesda, Md., USA for the NPY probe. C. A. was the recipient of scholarships from the Thai Government and Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand.