Journal article

ARGININE VASOPRESSIN AND CORTICOTROPIN RELEASING-FACTOR - BINDING TO OVINE ANTERIOR-PITUITARY MEMBRANES

PJ SHEN, IJ CLARKE, BJ CANNY, JW FUNDER, AI SMITH

ENDOCRINOLOGY | ENDOCRINE SOC | Published : 1990

Abstract

In the sheep, in contrast to the rat, arginine vasopressin (AVP) is a more potent stimulus to ACTH secretion from the anterior pituitary (AP) than CRF. To further explore this difference, we have compared [3H]AVP and [125I]-[Nle21 Tyr32]ovine CRF binding in membranes prepared from rat and sheep AP. Between species, no difference in affinity of binding was found for either ligand. In contrast, the concentration of AVP receptors in sheep AP was twice that in rat, whereas that of CRF receptors was only one tenth. AVP receptor concentration in sheep AP was not altered by chronic (10 day) dexamethasone administration, but fell to 60% of control after chronic (60 day) hypothalamo-pituitary-disconn..

View full abstract

University of Melbourne Researchers