Journal article

Opposite fates of the purine metabolite allantoin under water and nitrogen limitations in bread wheat

Alberto Casartelli, Vanessa J Melino, Ute Baumann, Matteo Riboni, Radoslaw Suchecki, Nirupama S Jayasinghe, Himasha Mendis, Mutsumi Watanabe, Alexander Erban, Ellen Zuther, Rainer Hoefgen, Ute Roessner, Mamoru Okamoto, Sigrid Heuer

PLANT MOLECULAR BIOLOGY | SPRINGER | Published : 2019

Abstract

KEY MESSAGE: Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleotides) and is known for its housekeeping role in nitrogen (N) recycling and also for its function in N transport and storage in nodulated legumes. Allantoin was also shown to differentially accumulate upon abiotic stress in a range of plant species but little is known about its role in cereals. To address this, purine catabolic pathway genes were identified in hexaploid bread w..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council


Awarded by Designing Future Wheat (DFW) Strategic Programme


Funding Acknowledgements

We thank Akiko Enju, Pia Muller and Jessey George (University of Adelaide) for supporting with sample collection; Juan Carlos Sanchez-Ferrero (University of Adelaide) for support with bioinformatic analyses; Adam Lukaszewski (University of Riverside, California, US) for providing the Chinese Spring NT seeds stock and Margaret Pallotta (University of Adelaide) for providing DNA of the NT lines; Sanjiv Satija, Larissa Chirkova and Yuan Li (University of Adelaide) for technical support with molecular analyses; Julian Taylor (University of Adelaide) for support in experimental design; Siria Natera, Gina Barossa and Veronica Liu (Metabolomics Australia) for support with the allantoin quantification. We are grateful to Elmien Heyneke, Ines Fehrle and Astrid Basner (Max Plank Institute, Potsdam-Golm) for their support in performing the metabolomics analysis. This work was supported by an Australian Research Council linkage Grant LP1400100239 in partnership with and additional funding from DuPont-Pioneer (USA). Rothamsted Research receives strategic funding from the Biotechnological and Biological Sciences Research Council of the United Kingdom. We acknowledge support through the Designing Future Wheat (DFW) Strategic Programme (BB/P016855/1).