Journal article

Genome-wide DNA methylation in saliva and body size of adolescent girls

Trine B Rounge, Christian M Page, Maija Lepisto, Pekka Ellonen, Bettina K Andreassen, Elisabete Weiderpass

EPIGENOMICS | FUTURE MEDICINE LTD | Published : 2016

Abstract

AIM: We performed an epigenome-wide association study within the Finnish Health in Teens cohort to identify differential DNA methylation and its association with BMI in adolescents. MATERIALS & METHODS: Differential DNA methylation analyses of 3.1 million CpG sites were performed in saliva samples from 50 lean and 50 heavy adolescent girls by genome-wide targeted bisulfite-sequencing. RESULTS: We identified 100 CpG sites with p-values < 0.000524, seven regions by 'bumphunting' and five CpG islands that differed significantly between the two groups. The ten CpG sites and regions most strongly associated with BMI substantially overlapped with obesity- and insulin-related genes, including MC2R,..

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University of Melbourne Researchers

Grants

Awarded by Folkhalsan Research Foundation: The Academy of Finland


Awarded by Life and Health Medical Fund


Awarded by Swedish Cultural Foundation in Finland


Awarded by Signe and Ane Gyllenberg Foundation


Awarded by Yrjo Jahnsson Foundation


Awarded by Academy of Finland (AKA)


Funding Acknowledgements

This work was supported by the Folkhalsan Research Foundation: The Academy of Finland (grant number 250704); The Life and Health Medical Fund (grant number 1-23-28); The Swedish Cultural Foundation in Finland (grant number 15/0897); The Signe and Ane Gyllenberg Foundation (grant number 37-1977-43); and The Yrjo Jahnsson Foundation (grant number 11486). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.