A long non-coding RNA promotes full activation of adult gene expression in the chicken alpha-globin domain
Cristian Arriaga-Canon, Yael Fonseca-Guzman, Christian Valdes-Quezada, Rodrigo Arzate-Mejia, Georgina Guerrero, Felix Recillas-Targa
EPIGENETICS | TAYLOR & FRANCIS INC | Published : 2014
Long non-coding RNAs (lncRNAs) were recently shown to regulate chromatin remodelling activities. Their function in regulating gene expression switching during specific developmental stages is poorly understood. Here we describe a nuclear, non-coding transcript responsive for the stage-specific activation of the chicken adult α(D) globin gene. This non-coding transcript, named α-globin transcript long non-coding RNA (lncRNA-αGT) is transcriptionally upregulated in late stages of chicken development, when active chromatin marks the adult α(D) gene promoter. Accordingly, the lncRNA-αGT promoter drives erythroid-specific transcription. Furthermore, loss of function experiments showed that lncRNA..View full abstract
Awarded by Direccion General de Asuntos del Personal Academico-Universidad Nacional Autonoma de Mexico
Awarded by Consejo Nacional de Ciencia y Tecnologia, Mexico (CONACyT)
We thank Paul Delgado-Olgun and Ricardo Saldana-Meyer for critical reading of the manuscript and suggestions. This work was supported by the Direccion General de Asuntos del Personal Academico-Universidad Nacional Autonoma de Mexico (IN209403 and IN203811), Consejo Nacional de Ciencia y Tecnologia, Mexico (CONACyT; 42653-Q and 128464). Ph.D. fellowship from CONACyT and Direccion General de Estudios de Posgrado-Universidad Nacional Autonoma de Mexico (DGEP) (Arriaga-Canon C, Valdes-Quezada C, Arzate-Mejia R and Fonseca-Guzman Y). Additional support was provided by the PhD Graduate Program, "Doctorado en Ciencias Biomedicas," to the Instituto de Fisiologia Celular and the Universidad Nacional Autonoma de Mexico. We thank the technical assistance of Fernando Suaste Olmos.