Journal article

Age-related methylation patterning of housekeeping genes and tissue-specific genes is distinct between the stomach antrum and body

Seung-Jin Hong, Hwa-Jeong Lee, Jung-Hwan Oh, Sung-Hoon Jung, Ki-Ouk Min, Sang-Wook Choi, Mun-Gan Rhyu



AIM: The methylation-variable sites around CpG islands are frequently overmethylated in Helicobacter pylori-infected stomachs. Age-related patterns of the overmethylation changes were compared between the fast-growing antrum cells and the slow-growing body cells. MATERIALS & METHODS: A total of 316 H. pylori-positive tissues and 380 H. pylori-negative tissues were obtained by endoscopic biopsy. The methylation-variable sites of ten housekeeping genes and nine tissue-specific genes were semiquantitatively analyzed, based on the ten-level classification of methylation-specific PCR intensity. The overmethylated genes were scored when their methylation levels were higher than an intermediate lev..

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Awarded by Catholic Medical Center Research Foundation

Funding Acknowledgements

This work was supported by the Catholic Medical Center Research Foundation made in the program year of 2011 (5-2011-B0001-00029). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.