Age-related methylation patterning of housekeeping genes and tissue-specific genes is distinct between the stomach antrum and body
Seung-Jin Hong, Hwa-Jeong Lee, Jung-Hwan Oh, Sung-Hoon Jung, Ki-Ouk Min, Sang-Wook Choi, Mun-Gan Rhyu
EPIGENOMICS | FUTURE MEDICINE LTD | Published : 2013
AIM: The methylation-variable sites around CpG islands are frequently overmethylated in Helicobacter pylori-infected stomachs. Age-related patterns of the overmethylation changes were compared between the fast-growing antrum cells and the slow-growing body cells. MATERIALS & METHODS: A total of 316 H. pylori-positive tissues and 380 H. pylori-negative tissues were obtained by endoscopic biopsy. The methylation-variable sites of ten housekeeping genes and nine tissue-specific genes were semiquantitatively analyzed, based on the ten-level classification of methylation-specific PCR intensity. The overmethylated genes were scored when their methylation levels were higher than an intermediate lev..View full abstract
Awarded by Catholic Medical Center Research Foundation
This work was supported by the Catholic Medical Center Research Foundation made in the program year of 2011 (5-2011-B0001-00029). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.