Journal article
Structure of an influenza a virus n9 neuraminidase with a tetrabrachion-domain stalk
VA Streltsov, PM Schmidta, JLMK Breschkina
Acta Crystallographica Section F Structural Biology Communications | INT UNION CRYSTALLOGRAPHY | Published : 2019
Abstract
The influenza neuraminidase (NA) is a homotetramer with head, stalk, transmembrane and cytoplasmic regions. The structure of the NA head with a stalk has never been determined. The NA head from an N9 subtype influenza A virus, A/tern/Australia/G70C/1975 (H1N9), was expressed with an artificial stalk derived from the tetrabrachion (TB) tetramerization domain from Staphylothermus marinus. The NA was successfully crystallized both with and without the TB stalk, and the structures were determined to 2.6 and 2.3 A ° resolution, respectively. Comparisons of the two NAs with the native N9 NA structure from egg-grown virus showed that the artificial TB stalk maintained the native NA head structure, ..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
Parts of this study were supported by grant R01A1062721 from the NIAID. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.