Yap is a novel regulator of C2C12 myogenesis
Kevin I Watt, Robert Judson, Paul Medlow, Kenneth Reid, Tobias B Kurth, Jatin G Burniston, Aivaras Ratkevicius, Cosimo De Bari, Henning Wackerhage
Biochemical and Biophysical Research Communications | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2010
The expression, regulation and function of mammalian Hippo pathway members in skeletal muscle is largely unknown. The aim of this study was thus to test the hypothesis that core members of the mammalian Hippo pathway are expressed in skeletal muscle and that the transcriptional co-factor Yap, a core member of the Hippo pathway, regulates C2C12 myogenesis. We found that the major components of the mammalian Hippo pathway including Yap are all expressed in skeletal muscles, C2C12 myoblasts and myotubes. In C2C12 myoblasts, Yap Ser127 phosphorylation is low and Yap localises to nuclei. Upon differentiation, Yap Ser127 phosphorylation increases approximately 20-fold and Yap translocates from the..View full abstract
Awarded by Medical Research Council
We are indebted to Dr. Marius SudoL, who created the DNA constructs used in this study and made them available via Addgene. We thank technicians Denise Tosh and Patricia Crombie for supporting these experiments. Robert Judson was funded by the Oliver Bird Rheumatism Programme. Prof. De Bari is a Fellow of the Medical Research Council, UK.