Journal article

Harmonization of lateral fluid-percussion injury model production and post-injury monitoring in a preclinical multicenter biomarker discovery study on post-traumatic epileptogenesis

Xavier Ekolle Ndode-Ekane, Cesar Santana-Gomez, Pablo M Casillas-Espinosa, Idrish Ali, Rhys D Brady, Gregory Smith, Pedro Andrade, Riikka Immonen, Noora Puhakka, Matthew R Hudson, Emma L Braine, Sandy R Shultz, Richard J Staba, Terence J O'Brien, Asla Pitkanen

EPILEPSY RESEARCH | ELSEVIER SCIENCE BV | Published : 2019

Abstract

Multi-center preclinical studies can facilitate the discovery of biomarkers of antiepileptogenesis and thus facilitate the diagnosis and treatment development of patients at risk of developing post-traumatic epilepsy. However, these studies are often limited by the difficulty in harmonizing experimental protocols between laboratories. Here, we assess whether the production of traumatic brain injury (TBI) using the lateral fluid-percussion injury (FPI) in adult male Sprague-Dawley rats (12 weeks at the time of injury) was harmonized between three laboratories - located in the University of Eastern Finland (UEF), Monash University in Melbourne, Australia (Melbourne) and The University of Calif..

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Grants

Awarded by National Institute of Neurological Disorders and Stroke (NINDS) Center without Walls of the National Institutes of Health (NIH)


Awarded by NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE


Funding Acknowledgements

This research was supported by the National Institute of Neurological Disorders and Stroke (NINDS) Center without Walls of the National Institutes of Health (NIH) under Award Number U54N8100064 (EpiBioS4Rx).