Postnatal germ cell development during first 18 months of life in testes from boys with non-syndromic cryptorchidism and complete or partial androgen insensitivity syndrome
Ruili Li, Damian Azzollini, Ruidong Shen, Jorgen Thorup, Erick Clasen-Linde, Dina Cortes, John M Hutson
Journal of Pediatric Surgery | W B SAUNDERS CO-ELSEVIER INC | Published : 2019
INTRODUCTION: Neonatal testicular germ cells/gonocytes, transform into stem cells for spermatogenesis during 'minipuberty', driving change in timing of surgery. This study examined gonocyte transformation in cryptorchid testes in children ≤18 months of age with unilateral, bilateral undescended testes (UDT), complete or partial androgen insensitivity syndrome (CAIS, PAIS) [3,4]. MATERIAL AND METHODS: Testicular biopsies were taken from patients with unilateral or bilateral UDT, PAIS or CAIS, aged 10 days-18 months. These testicular sections underwent immunohistochemistry with antibodies (Oct4, Ki67, C-Kit, Sox9) followed by confocal imaging, cell counting and statistical analysis. RESULTS: B..View full abstract
Awarded by Australian National Health and Medical Research Council
This study was supported by the Australian National Health and Medical Research Council (Grant Number APP1144752) and the Victorian Government's Operational Infrastructure Support Programme.