Journal article

The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family

Kathryn ER Parker, Stephen J Fairweather, Esther Rajendran, Martin Blume, Malcolm J McConville, Stefan Broer, Kiaran Kirk, Giel G van Dooren

PLOS PATHOGENS | PUBLIC LIBRARY SCIENCE | Published : 2019

Abstract

Apicomplexan parasites are auxotrophic for a range of amino acids which must be salvaged from their host cells, either through direct uptake or degradation of host proteins. Here, we describe a family of plasma membrane-localized amino acid transporters, termed the Apicomplexan Amino acid Transporters (ApiATs), that are ubiquitous in apicomplexan parasites. Functional characterization of the ApiATs of Toxoplasma gondii indicate that several of these transporters are important for intracellular growth of the tachyzoite stage of the parasite, which is responsible for acute infections. We demonstrate that the ApiAT protein TgApiAT5-3 is an exchanger for aromatic and large neutral amino acids, w..

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Grants

Awarded by Australian Research Council (ARC)


Funding Acknowledgements

This work was supported by a Discovery grant from the Australian Research Council (ARC) to KK, GGvD, and SB (DP150102883). SJF was supported by the Phyllis Montgomerie Award from the Royal Commonwealth Society. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.