Journal article

Macrophages, rather than DCs, are responsible for inflammasome activity in the GM-CSF BMDC model

Ziv Erlich, Inbar Shlomovitz, Liat Edry-Botzer, Hadar Cohen, Daniel Frank, Hanging Wang, Andrew M Lew, Kate E Lawlor, Yifan Zhan, James E Vince, Motti Gerlic

NATURE IMMUNOLOGY | NATURE PUBLISHING GROUP | Published : 2019

Abstract

Inflammasomes are one of the most important mechanisms for innate immune defense against microbial infection but are also known to drive various inflammatory disorders via processing and release of the cytokine IL-1β. As research into the regulation and effects of inflammasomes in disease has rapidly expanded, a variety of cell types, including dendritic cells (DCs), have been suggested to be inflammasome competent. Here we describe a major fault in the widely used DC-inflammasome model of bone marrow-derived dendritic cells (BMDCs) generated with the cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF). We found that among GM-CSF bone marrow-derived cell populations, monocyte-..

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Grants

Awarded by Israel Science Foundation (ISF)


Awarded by National Health and Medical Research Council (NHMRC) of Australia


Awarded by NHMRC


Funding Acknowledgements

This work was performed in partial fulfillment of the requirements for a Ph.D. degree (for Z.E. and I.S.), the Sackler Faculty of Medicine, Tel Aviv University, Israel. The research of M.G. was supported by the Israel Science Foundation (ISF) (grants 1416/15 and 818/18), alpha-1 Foundation, Recanati Foundation (Tel Aviv University) and individual research grants from the Varda and Boaz Dotan Research Center. The research of Y.Z. and A. M. L. was supported by the National Health and Medical Research Council (NHMRC) of Australia (grants 1037321, 1105209, 1080321 and 1143976). This work was also made possible by NHMRC project grants 1101405 (J.E.V.) and 1145788 (J.E.V. and K. E.L.) and NHMRC Fellowship 1141466 (J.E.V.).