Journal article

A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation

Bart A Westerman, A Koen Braat, Nicole Taub, Marko Potman, Joseph HA Vissers, Marleen Blom, Els Verhoeven, Hans Stoop, Ad Gillis, Arno Velds, Wouter Nijkamp, Roderick Beijersbergen, Lukas A Huber, Leendert HJ Looijenga, Maarten van Lohuizen

Journal of Experimental Medicine | ROCKEFELLER UNIV PRESS | Published : 2011

Abstract

Despite intense investigation of intrinsic and extrinsic factors that regulate pluripotency, the process of initial fate commitment of embryonic stem (ES) cells is still poorly understood. We used a genome-wide short hairpin RNA screen in mouse ES cells to identify genes that are essential for initiation of differentiation. Knockdown of the scaffolding protein Mek binding protein 1 (Mp1, also known as Lamtor3 or Map2k1ip1) stimulated self-renewal of ES cells, blocked differentiation, and promoted proliferation. Fibroblast growth factor 4 (FGF4) signaling is required for initial fate commitment of ES cells. Knockdown of Mp1 inhibited FGF4-induced differentiation but did not alter FGF4-driven ..

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University of Melbourne Researchers