Replica moulded poly(dimethylsiloxane) microwell arrays induce localized endothelial cell immobilization for coculture with pancreatic islets
Anouck LS Burzava, Aurelien Forget, Frances J Harding, Michaelia P Cockshell, Daniella Penko, Camille Rouzaud, Vincent Ahmadi, Paula F Marina, Darling Rojas-Canales, Claudine S Bonder, P Toby H Coates, Michaela Waibel, Helen E Thomas, Thomas W Kay, Thomas Loudovaris, Anton Blencowe, Nicolas H Voelcker
BIOINTERPHASES | AMER INST PHYSICS | Published : 2019
PolyJet three-dimensional (3D) printing allows for the rapid manufacturing of 3D moulds for the fabrication of cross-linked poly(dimethylsiloxane) microwell arrays (PMAs). As this 3D printing technique has a resolution on the micrometer scale, the moulds exhibit a distinct surface roughness. In this study, the authors demonstrate by optical profilometry that the topography of the 3D printed moulds can be transferred to the PMAs and that this roughness induced cell adhesive properties to the material. In particular, the topography facilitated immobilization of endothelial cells on the internal walls of the microwells. The authors also demonstrate that upon immobilization of endothelial cells ..View full abstract
This work was funded by the Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM). This work was performed in part at the South Australian and Victorian nodes of the Australian National Fabrication Facility under the National Collaborative Research Infrastructure Strategy. St Vincent's Institute receives support from the Operational Infrastructure Support Scheme of the Government of Victoria. The authors would like to thank Marta Krasowska for help with AFM analysis.