Journal article

Novel Cre-Expressing Mouse Strains Permitting to Selectively Track and Edit Type 1 Conventional Dendritic Cells Facilitate Disentangling Their Complexity in vivo

Raphael Mattiuz, Christian Wohn, Sonia Ghilas, Marc Ambrosini, Yannick O Alexandre, Cindy Sanchez, Anissa Fries, Vu Manh Thien-Phong, Bernard Malissen, Marc Dalod, Karine Crozat

Frontiers in Immunology | FRONTIERS MEDIA SA | Published : 2018

Abstract

Type 1 conventional DCs (cDC1) excel in the cross-priming of CD8+ T cells, which is crucial for orchestrating efficient immune responses against viruses or tumors. However, our understanding of their physiological functions and molecular regulation has been limited by the lack of proper mutant mouse models allowing their conditional genetic targeting. Because the Xcr1 and A530099j19rik (Karma/Gpr141b) genes belong to the core transcriptomic fingerprint of mouse cDC1, we used them to engineer two novel Cre-driver lines, the Xcr1 Cre and Karma Cre mice, by knocking in an IRES-Cre expression cassette into ..

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Grants

Awarded by European Research Council under the European Community's Seventh Framework Program


Awarded by Fondation pour la Recherche Medicale


Awarded by Program Hubert Curien (PHC) Maimonide-Israel 2017 project - French Ministry of Foreign Affairs (MAEDI)


Awarded by French Ministry of Research via PHENOMIN (French National Infrastructure for mouse Phenogenomics)


Funding Acknowledgements

This work was in part carried out in the frame of the Innate Immunocytes in Health and Disease (I2HD) collaborative project between CIML, AVIESAN, and SANOFI. It was supported by grants from the European Research Council under the European Community's Seventh Framework Program [FP7/2007-2013 grant agreement number 281225 to M. Dalod; FP7/2007-2013 grant no. 322465 (Integrate) to BM], from the Agence Nationale de la Recherche (ANR; XCR1-DirectingCells to KC), from the Fondation pour la Recherche Medicale (label Equipe FRM 2011, project number DEQ20110421284 to MD), which supported the Karma<SUP>Cre</SUP> mouse generation, from the Program Hubert Curien (PHC) Maimonide-Israel 2017 project number 38155SJ (to MD) supported by the French Ministries of Foreign Affairs (MAEDI) and of Research and Higher Education (MENESR) and from the Fondation ARC pour la recherche sur le cancer (to KC). CIPHE is supported by the French Ministry of Research via PHENOMIN (French National Infrastructure for mouse Phenogenomics; ANR10-INBS-07 to BM). This work also benefited from institutional funding from CNRS and INSERM. RM was supported by doctoral fellowships from the Biotrail PhD program (Fondation A*MIDEX). RM, CW, SG, and YA were supported by fellowships from Fondation ARC pour la recherche sur le cancer.