Retinal metabolic events in preconditioning light stress as revealed by wide-spectrum targeted metabolomics
Juan Manuel Chao de la Barca, Nuan-Ting Huang, Haihan Jiao, Lydie Tessier, Cedric Gadras, Gilles Simard, Riccardo Natoli, Guillaume Tcherkez, Pascal Reynier, Krisztina Valter
METABOLOMICS | SPRINGER | Published : 2017
INTRODUCTION: Light is the primary stimulus for vision, but may also cause damage to the retina. Pre-exposing the retina to sub-lethal amount of light (or preconditioning) improves chances for retinal cells to survive acute damaging light stress. OBJECTIVES: This study aims at exploring the changes in retinal metabolome after mild light stress and identifying mechanisms that may be involved in preconditioning. METHODS: Retinas from 12 rats exposed to mild light stress (1000 lux × for 12 h) and 12 controls were collected one and seven days after light stress (LS). One retina was used for targeted metabolomics analysis using the Biocrates p180 kit while the fellow retina was used for histologi..View full abstract
Awarded by Australian Research Council
Awarded by NATIONAL EYE INSTITUTE
This work was funded by "Berthe Fouassier" fellowship from the Fondation de France (grant allocated to JMCB). We also acknowledge the support from the INSERM, CNRS, the University-Hospital of Angers, the Region Pays de la Loire and Angers Loire Metropole. This work was also supported by grants from the following patients' foundations: Fondation VISIO, Ouvrir les Yeux, Union Nationale des Aveugles et Deficients Visuels, Association contre les Maladies Mitochondriales, Retina France, Association Point de Mire and Association de Specialisation et d'Orientation Scientifique. GT acknowledges the support of the Australian Research Council via a Future Fellowship, under contract FT140100645.