The companion of cellulose synthase 1 confers salt tolerance through a Tau-like mechanism in plants
Christopher Kesten, Arndt Wallmann, Rene Schneider, Heather E McFarlane, Anne Diehl, Ghazanfar Abbas Khan, Barth-Jan van Rossum, Edwin R Lampugnani, Witold G Szymanski, Nils Cremer, Peter Schmieder, Kristina L Ford, Florian Seiter, Joshua L Heazlewood, Clara Sanchez-Rodriguez, Hartmut Oschkinat, Staffan Persson
Nature Communications | NATURE PUBLISHING GROUP | Published : 2019
Awarded by ARC
Awarded by Hermon-Slade Grant
Awarded by ARC DECRA
Awarded by SNF grant
Live cell imaging was performed with equipment maintained by the Center for Microscopy and Image Analysis (University of Zurich) and Scientific Center for Optical and Electron Microscopy (ScopeM, ETH Zurich). We thank Martina Leidert and Natalja Erdmann for help with protein expression and purification and Ines Kretschmar for synthesizing the peptides. We thank Dmytro Puchkov for performing the MT quality control by EM and Tim Scholz (Hannover Medical School) for assistance with the microtubule purification protocol. We also thank the Diez group, especially Felix Ruhnow, (B CUBE Center for Molecular Bioengineering, Dresden) for assistance with microtubule imaging and in vitro assays. The helper plasmid pBAD-sigma 32 (I54N) was a gift from Jeffery Kelly<SUP>73</SUP> (Addgene plasmid #59982), pETM11 was kindly supplied by EMBL Protein Production facility (Heidelberg Germany). We thank the Biological Optical Microscopy Platform, the Melbourne Advanced Microscopy Facility, and the Mass Spectrometry and Proteomics Facility (School of Biosciences and Bio21) at the University of Melbourne. S.P. was supported by an ARC Discovery grant (DP190101941), a Hermon-Slade Grant (Persson HSF 15/4) and a Future Fellowship grant (FT160100218). J.L.H. was supported by an ARC Future Fellowship (FT130101165). H.E.M. was supported by an ARC DECRA (DE170100054). C.S.R. and C.K. were supported by ETHZ and a SNF grant (2-77212-15). R.S. received Computational Biology Research Initiative and Early Career Research Grants from the University of Melbourne. C.K. was supported by a Peter und Traudl Engelhorn-Stiftung fellowship.