Journal article

Evaluation of sterilisation methods for bio-ink components: gelatin, gelatin methacryloyl, hyaluronic acid and hyaluronic acid methacryloyl

Cathal D O'Connell, Carmine Onofrillo, Serena Duchi, Xin Li, Yifan Zhang, Peilin Tian, Lanyita Lu, Anna Trengove, Anita Quigley, Sanjeev Gambhir, Afsaneh Khansari, Tajanka Mladenovska, Andrea O'Connor, Claudia Di Bella, Peter F Choong, Gordon G Wallace

Biofabrication | IOP PUBLISHING LTD | Published : 2019

Abstract

Reliable and scalable sterilisation of hydrogels is critical to the clinical translation of many biofabrication approaches, such as extrusion-based 3D bioprinting of cell-laden bio-inks. However sterilisation methods can be destructive, and may have detrimental effects on the naturally-derived hydrogels that constitute much of the bio-ink palette. Determining effective sterilisation methods requires detailed analysis of the effects of sterilisation on relevant properties such as viscosity, printability and cytocompatibility. Yet there have been no studies specifically exploring the effects of sterilisation on bio-inks to date. In this work, we explored the effects of various sterilisation te..

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