Localization of Outer Membrane Proteins in Treponema denticola by Quantitative Proteome Analyses of Outer Membrane Vesicles and Cellular Fractions
Paul D Veith, Michelle D Glew, Dhana G Gorasia, Dina Chen, Neil M O'Brien-Simpson, Eric C Reynolds
JOURNAL OF PROTEOME RESEARCH | AMER CHEMICAL SOC | Published : 2019
The identification and localization of outer membrane proteins (Omps) and lipoproteins in pathogenic treponemes such as T. denticola (periodontitis) and T. pallidum (syphilis) has been challenging. In this study, label-free quantitative proteomics using MaxQuant was applied to naturally produced outer membrane vesicles (OMVs) and cellular fractions to identify 1448 T. denticola proteins. Of these, 90 proteins were localized to the outer membrane (OM) comprising 59 lipoproteins, 25 β-barrel proteins, and six other putative OM-associated proteins. Twenty-eight lipoproteins were localized to the inner membrane (IM), and 43 proteins were assigned to the periplasm. The signal cleavage regions of ..View full abstract
Awarded by Australian Government Department of Industry, Innovation and Science
This research was supported by the Australian Government Department of Industry, Innovation and Science Grant ID 20080108. The authors thank Mr. William Singleton for the provision of cells and Dr. Jessica Cecil for the provision of both cells and purified OMV samples. The authors thank Dr. Shuai Nie, Dr. Ching-Seng Ang and Dr. Nicholas Williamson for the acquisition of Orbitrap LC-MS/MS data and their technical support through the Mass Spectrometry and Proteomics Facility at Bio21 Institute, The University of Melbourne, Australia.