Journal article

Adult rat retinal ganglion cells and glia can be printed by piezoelectric inkjet printing

Barbara Lorber, Wen-Kai Hsiao, Ian M Hutchings, Keith R Martin

Biofabrication | IOP PUBLISHING LTD | Published : 2014

Abstract

We have investigated whether inkjet printing technology can be extended to print cells of the adult rat central nervous system (CNS), retinal ganglion cells (RGC) and glia, and the effects on survival and growth of these cells in culture, which is an important step in the development of tissue grafts for regenerative medicine, and may aid in the cure of blindness. We observed that RGC and glia can be successfully printed using a piezoelectric printer. Whilst inkjet printing reduced the cell population due to sedimentation within the printing system, imaging of the printhead nozzle, which is the area where the cells experience the greatest shear stress and rate, confirmed that there was no ev..

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Grants

Awarded by van Geest Fight for Sight Early Career Investigator Award


Awarded by EPSRC


Awarded by Engineering and Physical Sciences Research Council


Awarded by Fight for Sight


Funding Acknowledgements

This work was supported by a van Geest Fight for Sight Early Career Investigator Award, grant number 1868 [BL], and the high-speed camera was provided by the EPSRC Engineering Instrument Pool in conjunction with EPSRC grant EP/H018913/1. The open access fee was paid through the University of Cambridge's RCUK block grant. The authors gratefully acknowledge Dr Stephen D Hoath for his support in analysing the high-speed video images.