Journal article

An ex vivo rat eye model to aid development of high-resolution retina imaging devices for rodents

Christian van Oterendorp, Keith R Martin, Jiang Jian Zhong, Luis Diaz-Santana

JOURNAL OF MODERN OPTICS | TAYLOR & FRANCIS LTD | Published : 2010

Abstract

High resolution in vivo retinal imaging in rodents is becoming increasingly important in eye research. Development of suitable imaging devices currently requires many lengthy animal procedures. We present an ex vivo rat model eye with fluorescently labelled retinal ganglion cells (RGC) and nerve fibre bundles that reduces the need for animal procedures while preserving key properties of the living rat eye. Optical aberrations and scattering of four model eyes and eight live rat eyes were quantified using a Shack-Hartmann sensor. Fluorescent images from RGCs were obtained using a prototype scanning laser ophthalmoscope. The wavefront aberration root mean square value without defocus did not s..

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Funding Acknowledgements

This work was partially funded by Deutsche Forschungsgemeinschaft, Germany (CvO) and the GSK Clinician-Scientist Programme (KM). We are very grateful to Natalie Bull for help during some of the data acquisition sessions. Luis Diaz-Santana was at Cambridge University during part of this work as a visiting academic during a sabbatical year. He is indebted to both City University and Cambridge University for support during this time. We thank E. Ribak for providing a Matlab(TM) routine with the implementation of the algorithm for localisation of the centroid of the SH spots.