Human lipoproteins as a vehicle for the delivery of beta-carotene and alpha-tocopherol to HepG2 cells.
KR Martin, G Loo, ML Failla
Proc Soc Exp Biol Med | Published : 1997
Highly differentiated human cell lines represent a useful in vitro model for the study of carotenoid uptake, metabolism, and function. Carotenoids are usually introduced into tissue culture media either in organic solvents or as micelles, whereas carotenoids are localized in lipoproteins in vivo. Initially, the stability of beta-carotene and alpha-tocopherol in micelles and human lipoproteins under standard tissue culture conditions was compared. Recovery of beta-carotene and alpha-tocopherol was 27% +/- 2% and 73% +/- 2%, respectively, after overnight incubation of micellar beta-carotene and alpha-tocopherol in serum-free medium without cells. This marked loss of beta-carotene was attenuate..View full abstract