Journal article
aac(6′)-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon
JM Bell, JD Turnidge, P Andersson
Antimicrobial Agents and Chemotherapy | Published : 2010
DOI: 10.1128/AAC.01476-09
Abstract
We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6′)-Ib-cr that is capable of distinguishing between the two genetic aac(6′)-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon. Copyright © 2010, American Society for Microbiology. All Rights Reserved.