Conference Proceedings
Evaluation of In Vivo CAR Transgene Levels in Relapsed/Refractory Pediatric and Young Adult ALL and Adult DLBCL Tisagenlecleucel-Treated Patients
Rakesh Awasthi, Karen Thudium Mueller, Gregory A Yanik, Constantine S Tam, Susana Rives, Joseph P McGuirk, Michael A Pulsipher, Michael W Boyer, Ulrich Jaeger, Andre Baruchel, Gary Douglas Myers, Hyatt Balke-Want, Stephen J Schuster, Heather Stefanski, Michael R Bishop, Edward R Waldron, Ozlem Anak, Abhijit Chakraborty, Eric Bleickardt, Stephane Wong Show all
BLOOD | AMER SOC HEMATOLOGY | Published : 2018
Abstract
Abstract Background Quantitative polymerase chain reaction (qPCR) is an analytical method that has been used to investigate the in vivo kinetics of chimeric receptor antigen (CAR) transgene following the infusion of tisagenlecleucel. B cell aplasia, likely an "on-target toxicity" of tisagenlecleucel, has been considered a measure of functional persistence. (Maude SL et al. Blood 2015;125(26):4017-4023) Although the CAR transgene can be detected in peripheral blood of tisagenlecleucel treated patients, it is unclear whether CAR transgene detection by qPCR could be reliably used to inform treatment decision in an individual patient. Methods ..
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