Journal article

Pre-analytical stability of FGF23 with the contemporary immunoassays

Niek F Dirks, Edward R Smith, Natasja M van Schoor, Marc G Vervloet, Mariette T Ackermans, Robert de Jonge, Annemieke C Heijboer

CLINICA CHIMICA ACTA | ELSEVIER SCIENCE BV | Published : 2019

Abstract

BACKGROUND: Several publications have reported on the pre-analytical stability of fibroblast growth factor 23 (FGF23) and some recommend coating blood collecting tubes with protease inhibitors, in order to prevent degradation. These recommendations are based on observations for a first generation assay for the measurement of intact FGF23. However, if this also applies for the contemporary immunoassays, and at what stage of pre-analysis, is unknown. METHODS: We reviewed data from these previous reports on the issue of FGF23 stability and complemented these findings with data from novel experiments. RESULTS: We concluded that the contemporary intact FGF23 assays by Immutopics, Kainos, Millipor..

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Grants

Awarded by Netherlands Organization for Scientific Research (NWO)


Funding Acknowledgements

The Longitudinal Aging Study Amsterdam is supported by a grant from the Netherlands Ministry of Health Welfare and Sports, Directorate of Long-Term Care. The data collection [in 2012-2013 and 2013-2014] was financially supported by the Netherlands Organization for Scientific Research (NWO) in the framework of the project "New Cohorts of young old in the 21st century" (file number 480-10-014).