Modulation of the CCR5 Receptor/Ligand Axis by Seminal Plasma and the Utility of In Vitro versus In Vivo Models
Jennifer A Juno, Kathleen M Wragg, Anne B Kristensen, Wen Shi Lee, Kevin J Selva, Renee M van der Sluis, Anthony D Kelleher, Benjamin R Bavinton, Andrew E Grulich, Sharon R Lewin, Stephen J Kent, Matthew S Parsons
JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2019
Sexual HIV-1 transmission occurs primarily in the presence of semen. Although data from macaque studies suggest that CCR5+ CD4+ T cells are initial targets for HIV-1 infection, the impact of semen on T cell CCR5 expression and ligand production remains inconclusive. To determine if semen modulates the lymphocyte CCR5 receptor/ligand axis, primary human T cell CCR5 expression and natural killer (NK) cell anti-HIV-1 antibody-dependent beta chemokine production was assessed following seminal plasma (SP) exposure. Purified T cells produce sufficient quantities of RANTES to result in a significant decline in CCR5bright T cell frequency following 16 h of SP exposure (P = 0.03). Meanwhile, NK cells..View full abstract
This work was funded by a project grant from the National Health and Medical Research Council (NHMRC) of Australia to M.S.P. Funding for the Opposites Attract Study was received from the National Health and Medical Research Council, Australia, amfAR, Foundation for AIDS Research, USA, ViiV Healthcare, UK, and Gilead Sciences, USA. J.A.J. and S.J.K. are funded by NHMRC fellowships. S.R.L. is an NHMRC practitioner fellow.