Journal article

CD1b presents self and Borrelia burgdorferi diacylglycerols to human T cells

Peter Reinink, Michael NT Souter, Tan-Yun Cheng, Tamara van Gorkom, Stefanie Lenz, Joanna Kubler-Kielb, Klemen Strle, Kristin Kremer, Steven FT Thijsen, Allen C Steere, Dale I Godfrey, Daniel G Pellicci, D Branch Moody, Ildiko Van Rhijn

EUROPEAN JOURNAL OF IMMUNOLOGY | WILEY | Published : 2019

Abstract

Lyme disease is a common multisystem disease caused by infection with a tick-transmitted spirochete, Borrelia burgdorferi and related Borrelia species. The monoglycosylated diacylglycerol known as B. burgdorferi glycolipid II (BbGL-II) is a major target of antibodies in sera from infected individuals. Here, we show that CD1b presents BbGL-II to human T cells and that the TCR mediates the recognition. However, we did not detect increased frequency of CD1b-BbGL-II binding T cells in the peripheral blood of Lyme disease patients compared to controls. Unexpectedly, mapping the T cell specificity for BbGL-II-like molecules using tetramers and activation assays revealed a concomitant response to C..

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Grants

Awarded by Nederlands Wetenschappelijk Onderzoek grant


Awarded by NIH


Awarded by National Health and Medical Research Council (NHMRC)


Awarded by Australian Research Council (ARC)


Awarded by NHMRC Senior Principal Research Fellowship


Awarded by NHMRC Career Development Fellowship


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by funding from Nederlands Wetenschappelijk Onderzoek grant 824.02.002 and the NIH (AI116604, AI04393). P.R. was supported by a Boehringer Ingelheim Fonds travel grant. M.N.T.S. was supported by an Australian Postgraduate Award. M.J.S., D.I.G., and D.G.P. are supported by the National Health and Medical Research Council (NHMRC, 1063587) and the Australian Research Council (ARC; CE140100011). D.I.G. is also supported by NHMRC Senior Principal Research Fellowship (1020770) and D.G.P. is supported by an NHMRC Career Development Fellowship (APP1144308).