Journal article
RNA-seq of Isolated Chromaffin Cells Highlights the Role of Sex-Linked and Imprinted Genes in Adrenal Medulla Development
Wing Hei Chan, Masayuki Komada, Toshiaki Fukushima, E Michelle Southard-Smith, Colin R Anderson, Matthew J Wakefield
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2019
Abstract
Adrenal chromaffin cells and sympathetic neurons synthesize and release catecholamines, and both cell types are derived from neural crest precursors. However, they have different developmental histories, with sympathetic neurons derived directly from neural crest precursors while adrenal chromaffin cells arise from neural crest-derived cells that express Schwann cell markers. We have sought to identify the genes, including imprinted genes, which regulate the development of the two cell types in mice. We developed a method of separating the two cell types as early as E12.5, using differences in expression of enhanced yellow fluorescent protein driven from the tyrosine hydroxylase gene, and th..
View full abstractRelated Projects (2)
Grants
Awarded by National Health and Medical Research Council
Awarded by EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
Awarded by NATIONAL CANCER INSTITUTE
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
Funding Acknowledgements
This work was supported by the National Health and Medical Research Council (Project 1063822). We thank Heather Young of the University of Melbourne for valuable comments on the manuscript; Jan Morgan of the University of Melbourne and Dennis Buehler at Vanderbilt University Medical Center for technical assistance in tissue dissection and isolation; Paul Lau and Matthew Burton of the Murdoch Children's Research Institute for technical assistance in FACS; Ellie Cho and Paul McMillan of the Biological Optical Microscopy Platform (the University of Melbourne) for their technical assistance in microscopy imaging; Kym Pham and Karey Cheong of the Melbourne Translation Genomics Platform (the University of Melbourne) for technical assistance in RNA sequencing. We also thank Marnie Blewitt of the Walter and Eliza Hall Institute of Medical Research for helpful discussions on the genomic imprinting and Andrew Allen of the University of Melbourne for sharing the TH-IRES-Cre transgenic mice.