Loss of Neogenin1 in human colorectal carcinoma cells causes a partial EMT and wound-healing response

V Chaturvedi; A Fournier-Level; HM Cooper; MJ Murray

Journal article

Loss of Neogenin1 in human colorectal carcinoma cells causes a partial EMT and wound-healing response

V Chaturvedi, A Fournier-Level, HM Cooper, MJ Murray

Scientific Reports | NATURE PORTFOLIO | Published : 2019

DOI: 10.1038/s41598-019-40886-y

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Abstract

Neogenin1 (NEO1) is a receptor of the Deleted in Colorectal Carcinoma (DCC)/Frazzled/UNC-40 family, which regulates axon guidance but can also stabilize epithelial adherens junctions. NEO1 and DCC are also tumor suppressors that can inhibit metastasis by acting as dependence receptors. Given the role of NEO1 in maintaining adherens junctions we tested whether loss of NEO1 also promoted metastasis via an epithelial mesenchymal transition (EMT). Loss of NEO1 disrupted zonula adherens but tight junctions were unaffected. Neo1-depleted epithelial cells exhibited a more migratory morphology, had reduced F-actin rich stress-fibres and more basal lamellipodia. Microtubule density was decreased whil..

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University of Melbourne Researchers

Michael Murray Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

The authors would like to thank Biological Optical Microscopy Platform at The University of Melbourne for use of the confocal microscope. We also thank Fionna Zhu for critical reading of the manuscript. This work was supported by the National Health and Medical Research Council, Australia project grant GNT1107123 to M.J.M.