Journal article

Development of a human skeletal micro muscle platform with pacing capabilities

Richard J Mills, Benjamin L Parker, Pauline Monnot, Elise J Needham, Celine J Vivien, Charles Ferguson, Robert G Parton, David E James, Enzo R Porrello, James E Hudson

BIOMATERIALS | ELSEVIER SCI LTD | Published : 2019

Abstract

Three dimensional engineered culture systems are powerful tools to rapidly expand our knowledge of human biology and identify novel therapeutic targets for disease. Bioengineered skeletal muscle has been recently shown to recapitulate many features of native muscle biology. However, current skeletal muscle bioengineering approaches require large numbers of cells, reagents and labour, limiting their potential for high-throughput studies. Herein, we use a miniaturized 96-well micro-muscle platform to facilitate semi-automated tissue formation, culture and analysis of human skeletal micro muscles (hμMs). Utilising an iterative screening approach we define a serum-free differentiation protocol t..

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Funding Acknowledgements

We used the Australian National Fabrication Facility Queensland Node for the fabrication of the micro-muscle platform molds. The authors acknowledge the use of the Australian Microscopy & Microanalysis Research Facility at the Center for Microscopy and Microanalysis at The University of Queensland. B.L.P., D.E.J., E.R.P., R.G.P. and J.E.H. are supported by Fellowships and Project Grants from the National Health and Medical Research Council, the National Heart Foundation, Stem Cells Australia, The University of Queensland or QIMR Berghofer Medical Research Institute. The Murdoch Children's Research Institute is supported by the Victorian Government's Operational Infrastructure Support Program. The contents of the published material are solely the responsibility of the authors and do not reflect the views of the funding bodies. R.J.M., E.R.P. and J.E.H. are named inventors on a provisional patent relating to the technology developed in this manuscript.