Journal article

Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondria! DNA-deficient cancer cells

Lan-Feng Dong, Jaromira Kovarova, Martina Bajzikova, Ayenachew Bezawork-Geleta, David Svec, Berwini Endaya, Karishma Sachaphibulkij, Ana R Coelho, Natasa Sebkova, Anna Ruzickova, An S Tan, Katarina Kluckova, Kristyna Judasova, Katerina Zamecnikova, Zuzana Rychtarcikova, Vinod Gopalan, Ladislav Andera, Margarita Sobol, Bing Yan, Bijay Pattnaik Show all

eLife | ELIFE SCIENCES PUBLICATIONS LTD | Published : 2017

Abstract

Recently, we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether the transfer of mtDNA involves whole mitochondria, we injected B16ρ0 mouse melanoma cells into syngeneic C57BL/6N su9-DsRed2 mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration. Additionally, knockdown of key mitochondrial complex I (N..

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Grants

Awarded by Fundacao para a Ciencia e a Tecnologia


Awarded by Council for Scientific and Industrial Research


Awarded by Czech Science Foundation


Awarded by Ministerstvo Skolstvi, Mladeze a Telovychovy


Awarded by Akademie ved Ceske republiky


Awarded by Australian Research Council


Funding Acknowledgements

Fundacao para a Ciencia e a Tecnologia SFRH/BD/103399/2014 Ana R CoelhoCancer Society of New Zealand An S Tan Michael V BerridgeGenesis Oncology Trust An S Tan Michael V BerridgeMalaghan Institute of Medical Research An S Tan Michael V BerridgeCouncil for Scientific and Industrial Research MLP5502 Anurag Agrawal Naveen Bhatraju Bijay PattnaikWellcome DBT India Alliance Senior Bijay Pattnaik Fellowship Naveen Bhatraju Anurag AgrawalCzech Science Foundation 16-12816S Jaroslav TruksaMinisterstvo Skolstvi, Mladeze a Telovychovy LM2011032 Radislav SedlacekAkademie ved Ceske republiky RVO 68378050 Radislav SedlacekCzech Science Foundation 14-05547S Katerina Dvorakova-HortovaCzech Science Foundation 16-22823S Jakub RohlenaCzech Science Foundation 17-20904S Jakub RohlenaCzech Science Foundation 16-12816S Jakub RohlenaAustralian Research Council DP150102820 Jiri NeuzilCzech Science Foundation 17-0192J Jiri NeuzilCzech Science Foundation 16-12719S Jiri NeuzilCzech Science Foundation 15-02203S Jiri NeuzilBIOCEV European Regional Development Jiri Neuzil