Journal article
Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
LF Dong, J Kovarova, M Bajzikova, A Bezawork-Geleta, D Svec, B Endaya, K Sachaphibulkij, AR Coelho, N Sebkova, A Ruzickova, AS Tan, K Kluckova, K Judasova, K Zamecnikova, Z Rychtarcikova, V Gopalan, L Andera, M Sobol, B Yan, B Pattnaik Show all
Elife | eLIFE SCIENCES PUBL LTD | Published : 2017
DOI: 10.7554/eLife.22187
Abstract
Recently, we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (r0 cells) is linked to the acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether the transfer of mtDNA involves whole mitochondria, we injected B16r0 mouse melanoma cells into syngeneic C57BL/6Nsu9-DsRed2 mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration. Additionally, knockdown of key mitochondrial complex I (NDUFV1) and complex II (SDHC) subunits by shRNA..
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Awarded by Cancer Society of New Zealand
Funding Acknowledgements
Fundacao para a Ciencia e a Tecnologia SFRH/BD/103399/2014 Ana R Coelhor Cancer Society of New Zealand An S Tan Michael V Berridger Genesis Oncology Trust An S Tan Michael V Berridger Malaghan Institute of Medical Research An S Tan Michael V Berridger Council for Scientific and Industrial Research MLP5502 Anurag Agrawal Naveen Bhatraju Bijay Pattnaikr Wellcome DBT India Alliance Senior Bijay Pattnaik Fellowship Naveen Bhatraju Anurag Agrawalr Czech Science Foundation 16-12816S Jaroslav Truksar Ministerstvo Skolstvi, Mladeze a Telovychovy LM2011032 Radislav Sedlacekr Akademie ved Ceske republiky RVO 68378050 Radislav Sedlacekr Czech Science Foundation 14-05547S Katerina Dvorakova-Hortovar Czech Science Foundation 16-22823S Jakub Rohlenar Czech Science Foundation 17-20904S Jakub Rohlenar Czech Science Foundation 16-12816S Jakub Rohlenar Australian Research Council DP150102820 Jiri Neuzilr Czech Science Foundation 17-0192J Jiri Neuzilr Czech Science Foundation 16-12719S Jiri Neuzilr Czech Science Foundation 15-02203S Jiri Neuzilr BIOCEV European Regional Development Jiri Neuzil