Journal article

Mitochondrial Genome Acquisition Restores Respiratory Function and Tumorigenic Potential of Cancer Cells without Mitochondrial DNA

An S Tan, James W Baty, Lan-Feng Dong, Ayenachew Bezawork-Geleta, Berwini Endaya, Jacob Goodwin, Martina Bajzikova, Jaromira Kovarova, Martin Peterka, Bing Yan, Elham Alizadeh Pesdar, Margarita Sobol, Anatolyj Filimonenko, Shani Stuart, Magdalena Vondrusova, Katarina Kluckova, Karishma Sachaphibulkij, Jakub Rohlena, Pavel Hozak, Jaroslav Truksa Show all

CELL METABOLISM | CELL PRESS | Published : 2015

Abstract

We report that tumor cells without mitochondrial DNA (mtDNA) show delayed tumor growth, and that tumor formation is associated with acquisition of mtDNA from host cells. This leads to partial recovery of mitochondrial function in cells derived from primary tumors grown from cells without mtDNA and a shorter lag in tumor growth. Cell lines from circulating tumor cells showed further recovery of mitochondrial respiration and an intermediate lag to tumor growth, while cells from lung metastases exhibited full restoration of respiratory function and no lag in tumor growth. Stepwise assembly of mitochondrial respiratory (super)complexes was correlated with acquisition of respiratory function. Our..

View full abstract

Grants

Awarded by Czech Science Foundation


Awarded by Technology Agency of the Czech Republic


Awarded by Ministry of Industry and Trade of the Czech Republic


Awarded by BIOCEV European Regional Development Fund


Funding Acknowledgements

We thank H. Matilainen for help with the orthotopic tumor model, J. Janacek for help with statistical analysis of the TEM data, and I. Wittig for critical reading of the manuscript. This research was supported by grants from Genesis Oncology Trust, the Cancer Society of New Zealand, the Breast Cancer Research Trust to M.V.B., grants from the National Health and Medical Research Council, Cancer Council Queensland, Clem Jones Foundation, and the Czech Science Foundation P301/10/1937 and GA15-02203S to J.N., a grant from the Australian Research Council to L.-F.D. and J.N., grants from the Czech Science Foundation P305/12/1708 to J.T. and P301/12/1851 to J.R., and grants from the Technology Agency of the Czech Republic (TE01020118) and Ministry of Industry and Trade of the Czech Republic (FR-TI3/588) to M.S., A.F., and P.H.L.-F.D. was supported in part by a Griffith University Senior Research Fellowship. This work was also supported by the Malaghan Institute, Australian Government EIF Super Science Funds as part of the Therapeutic Innovation Australia Queensland Node project to L.R.G., as well as by the BIOCEV European Regional Development Fund CZ.1.05/1.1.00/02.0109.