Journal article
Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice
RR Chaudhuri, SE Peters, SJ Pleasance, H Northen, C Willers, GK Paterson, DB Cone, AG Allen, PJ Owen, G Shalom, DJ Stekel, IG Charles, DJ Maskell
Plos Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2009
Abstract
Genes required for infection of mice by Salmonella Typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. Inactivation of such genes produces attenuated S. Typhimurium strains that have potential for use as live attenuated vaccines. A quantitative screen, Transposon Mediated Differential Hybridisation (TMDH), has been developed that identifies those members of a large library of transposon mutants that are attenuated. TMDH employs custom transposons with outward-facing T7 and SP6 promoters. Fluorescently-labelled transcripts from the promoters are hybridised to whole-genome tiling microarrays, to allow the position of..
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Awarded by BBSRC
Funding Acknowledgements
This work was supported by BBSRC grant numbers APG19115 and BBD0179471 (http://www.bbsrc.ac.uk) and Arrow Therapeutics Ltd. (http://www.arrowt.co.uk). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.