Journal article

Bordetella pertussis risA, but not risS, is required for maximal expression of Bvg-repressed genes

TH Stenson, AG Allen, JA al-Meer, D Maskell, MS Peppler

INFECTION AND IMMUNITY | AMER SOC MICROBIOLOGY | Published : 2005

Abstract

Expression of virulence determinants by Bordetella pertussis, the primary etiological agent of whooping cough, is regulated by the BvgAS two-component regulatory system. The role of a second two-component regulatory system, encoded by risAS, in this process is not defined. Here, we show that mutation of B. pertussis risA does not affect Bvg-activated genes or proteins. However, mutation of risA resulted in greatly diminished expression of Bvg-repressed antigens and decreased transcription of Bvg-repressed genes. In contrast, mutation of risS had no effect on the expression of Bvg-regulated molecules. Mutation of risA also resulted in decreased bacterial invasion in a HeLa cell model. However..

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University of Melbourne Researchers