Journal article

Mutagenesis of Streptococcus equi and Streptococcus suis by transposon Tn917

JD Slater, AG Allen, JP May, S Bolitho, H Lindsay, DJ Maskell

VETERINARY MICROBIOLOGY | ELSEVIER SCIENCE BV | Published : 2003

Abstract

Genetic tools for studying streptococci are much less sophisticated than those that are available for many other bacterial genera. In this paper, we describe the development of a transposon mutagenesis system that we have used to mutate two important veterinary streptococci, Streptococcus equi and Streptococcus suis. The system uses a temperature-sensitive suicide vector to deliver Tn917 via electroporation, transposing Tn917 into the chromosomal DNA of the two streptococci. The transposon insertions can be rescued from the streptococcal chromosomes by plasmid rescue and selection in E. coli, with subsequent insertion site analysis by DNA sequencing. Transposition appeared to have occurred i..

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