Journal article

A Pneumocystis carinii multi-gene family with homology to subtilisin-like serine proteases

EB Lugli, AG Allen, AE Wakefield

MICROBIOLOGY-UK | SOC GENERAL MICROBIOLOGY | Published : 1997

Abstract

Copies of multi-gene family, named PRT1 (protease 1), encoding a subtilisin-like serine protease were cloned from the opportunistic fungal pathogen Pneumocystis carinii. Comparison of the nucleotide sequence of a genomic clone and a cDNA clone of PRT1 from P. carinii f. sp. carinii revealed the presence of seven short introns. Several different domains were predicted from the deduced amino acid sequence: an N-terminal hydrophobic signal sequence, a pro-domain, a subtilisin-like catalytic domain, a P-domain (essential for proteolytic activity), a proline-rich domain, a serine/threonine-rich domain and a C-terminal hydrophobic domain. The catalytic domain showed high homology to other eukaryot..

View full abstract

University of Melbourne Researchers