Journal article

Generation of Vestibular Tissue-Like organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture System

Cristiana Mattei, Rebecca Lim, Hannah Drury, Babak Nasr, Zihui Li, Melissa A Tadros, Giovanna M D'Abaco, Kathryn S Stok, Bryony A Nayagam, Mirella Dottori

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | FRONTIERS MEDIA SA | Published : 2019

Abstract

Hair cells are specialized mechanosensitive cells responsible for mediating balance and hearing within the inner ear. In mammals, hair cells are limited in number and do not regenerate. Human pluripotent stem cells (hPSCs) provide a valuable source for deriving human hair cells to study their development and design therapies to treat and/or prevent their degeneration. In this study we used a dynamic 3D Rotary Cell Culture System (RCCS) for deriving inner ear organoids from hPSCs. We show RCCS-derived organoids recapitulate stages of inner ear development and give rise to an enriched population of hair cells displaying vestibular-like morphological and physiological phenotypes, which resemble..

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Grants

Awarded by Australian Research Council Future Fellowship


Awarded by NHMRC


Funding Acknowledgements

This study was supported by the University of Melbourne and Melbourne International Research Scholarship (CM), Australian Research Council Future Fellowship (MD; FT130100514), Garnett Passe and Rodney William Memorial Foundation Research Scholarship (BAN), ARC Stem Cells Australia and NHMRC Project Grant (RL; APP1048232). The helium ion microscopy and confocal microscopy were performed at the Materials Characterization and Fabrication Platform (MCFP) at the University of Melbourne.