Journal article
Circadian and wake-dependent changes in human plasma polar metabolites during prolonged wakefulness: A preliminary analysis
LK Grant, S Ftouni, B Nijagal, DP De Souza, D Tull, MJ McConville, SMW Rajaratnam, SW Lockley, C Anderson
Scientific Reports | NATURE PORTFOLIO | Published : 2019
Abstract
Establishing circadian and wake-dependent changes in the human metabolome are critical for understanding and treating human diseases due to circadian misalignment or extended wake. Here, we assessed endogenous circadian rhythms and wake-dependent changes in plasma metabolites in 13 participants (4 females) studied during 40-hours of wakefulness. Four-hourly plasma samples were analyzed by hydrophilic interaction liquid chromatography (HILIC)-LC-MS for 1,740 metabolite signals. Group-averaged (relative to DLMO) and individual participant metabolite profiles were fitted with a combined cosinor and linear regression model. In group-level analyses, 22% of metabolites were rhythmic and 8% were li..
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Funding Acknowledgements
We thank the study participants, and the technicians, staff and students of the Monash University Sleep and Circadian Medicine Laboratory who aided data collection. We also thank Caroline Beatty for recruitment and research support; William McMahon and Jinny Collett for research assistance in the study; the study physicians Dr Simon Joosten and Dr Eric Kuo; Sylvia Nguyen for conducting psychiatric screening; Veronica Lui for assistance with metabolomics sample extraction; Dr Shadab Rahman for assistance with statistical analysis; and Kellie Hamill for logistical help. The authors also acknowledge the assistance of the Adelaide Research Assay Facility for providing plasma melatonin analysis, and Dr Saravanan Dayalan and Sean O'Callaghan at Metabolomics Australia for assistance with metabolomics analyses. This research was supported by the CRC for Alertness, Safety, and Productivity.