Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction
Cyril Seillet, Gabrielle T Belz, J Moll (ed.), S Carotta (ed.)
TARGET IDENTIFICATION AND VALIDATION IN DRUG DISCOVERY, 2 EDITION | HUMANA PRESS INC | Published : 2019
Retroviral transduction is commonly used to modulate gene expression and is a powerful approach to understand the role of a gene using gain- or loss-of-function strategies. Retroviral vectors can stably integrate non-viral genes into host genomes, providing long-term modulation of gene expression in infected cells and their progeny. Here we describe the generation of retroviral supernatants and the steps to efficiently transduce genes in innate lymphoid cell (ILC) progenitors for subsequent analysis of ILC populations in vivo.
This work was supported by grants (Grants 1122277, 1098832) and fellowships from the National Health and Medical Research Council of Australia (CS and GTB) and the Australian Research Council (GTB). This study was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC Independent Research Institute Infrastructure.