Journal article

Loss of the SPHF Homologue Slr1768 Leads to a Catastrophic Failure in the Maintenance of Thylakoid Membranes in Synechocystis sp PCC 6803

Samantha J Bryan, Nigel J Burroughs, Carol Evered, Joanna Sacharz, Anja Nenninger, Conrad W Mullineaux, Edward M Spence



BACKGROUND: In cyanobacteria the photosystems are localised to, and maintained in, specialist membranes called the thylakoids. The mechanism driving the biogenesis of the thylakoid membranes is still an open question, with only two potential biogenesis factors, Vipp1 and Alb3 currently identified. METHODOLOGY/PRINCIPAL FINDINGS: We generated a slr1768 knockout using the pGEM T-easy vector and REDIRECT. By comparing growth and pigment content (chlorophyll a fluoresence) of the Δslr1768 mutant with the wild-type, we found that Δslr1768 has a conditional phenotype; specifically under high light conditions (130 µmol m(-2) s(-1)) thylakoid biogenesis is disrupted leading to cell death on a scale ..

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Awarded by BBSRC

Awarded by Biotechnology and Biological Sciences Research Council

Funding Acknowledgements

University of Warwick (, BBSRC grant no. BB/G021856/1 ( The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.