Muscle Antioxidant Enzymes Activity and Gene Expression Are Altered by Diet-Induced Increase in Muscle Essential Fatty Acid (-linolenic acid) Concentration in Sheep Used as a Model
Eric N Ponnampalam, Vahid Vahedi, Khageswor Giri, Paul Lewandowski, Joe L Jacobs, Frank R Dunshea
NUTRIENTS | MDPI | Published : 2019
This study investigated the effect of dietary manipulations on muscle fatty acid composition, the activities and relative mRNA expressions of antioxidant enzymes and the relationship between muscle enzyme activity or mRNA expression and alpha linolenic acid (ALA) concentration in sheep. Eighty-four lambs blocked on liveweight were randomly allocated to four dietary treatments, lucerne pasture (Lucerne), annual ryegrass pasture (Ryegrass), feedlot pellets (Feedlot) or annual ryegrass plus feedlot pellets (RyeFeedlot). After six weeks of feeding, lambs were slaughtered and within 30 min post-mortem, samples collected from the longissimus lumborum (LL) muscle for RNA isolation and measurement o..View full abstract
The funds for the slaughter of lambs, muscle sample collection, analyses of muscle for biochemical components and statistical analyses of data were supported by Meat and Livestock Australia (MLA)/Australian Meat Processor Corporation (AMPC) and Victorian Government (DJPR).