Structural and Functional Characterization of an Orphan ATP-Binding Cassette ATPase Involved in Manganese Utilization and Tolerance in Leptospira spp.
Nadia Benaroudj, Frederick Saul, Jacques Bellalou, Isabelle Miras, Patrick Weber, Vincent Bondet, Gerald L Murray, Ben Adler, Paula Ristow, Helene Louvel, Ahmed Haouz, Mathieu Picardeau
Journal of Bacteriology | AMER SOC MICROBIOLOGY | Published : 2013
Pathogenic Leptospira species are the etiological agents of the widespread zoonotic disease leptospirosis. Most organisms, including Leptospira, require divalent cations for proper growth, but because of their high reactivity, these metals are toxic at high concentrations. Therefore, bacteria have acquired strategies to maintain metal homeostasis, such as metal import and efflux. By screening Leptospira biflexa transposon mutants for their ability to use Mn(2+), we have identified a gene encoding a putative orphan ATP-binding cassette (ABC) ATPase of unknown function. Inactivation of this gene in both L. biflexa and L. interrogans strains led to mutants unable to grow in medium in which iron..View full abstract
Awarded by French Ministry of Research
P.R. was supported by CAPES (Brasil) and a Fiocruz-Pasteur grant. H.L. was supported by a grant from the Region Ile-de-France. G. L. M. was a recipient of an NHMRC Peter Doherty Fellowship. Aspects of the work were supported by the Australian Research Council and the National Health and Medical Research Council, the Institut Pasteur, and the French Ministry of Research (ANR-08-MIE-018).